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What is CryoEM?
CryoEM (coupled with single particle analysis) has become a popular method for high resolution 3D protein structure determination. An aqueous biological sample is frozen rapidly and irradiated with a beam of electrons from a transmission electron microscope. A detector senses how the electrons are scattered and computerized image processing techniques are used to reconstruct the 3D-shape of the molecule.
Why do you need CryoEM Services?
X-ray crystallography and NMR have traditionally been the methods of choice for high resolution 3D protein structure determination, but cryoEM has emerged as a complementary technique. It is well suited for targets that are not suitable for X-ray crystallography or NMR.
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